LUPUS ANTICOAGULANT (LAC)

Patient Preparation:"Note 1: Preparation of PPP : 12 -14 Hrs. (Overnight) fasting is preferred. Centrifuge the blood sample at 3000rpm for 10 minutes within 1 hr. of collection to separate the plasma. Transfer the Plasma obtained to a clean plastic tube using a plastic tipped pipette avoiding buffy coat & centrifuge it again at 3700 rpm & finally transfer the PPP into fresh, clean & labelled plastic tubes which contains <10,000 cells/cu. mm. Note 2: Discontinue Heparin for 1 day and oral anti coagulants for 7 days prior to sampling. Discontinuation should be with prior consent from treating physician"